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目录3

第一部分 造血干细胞3

我国造血干细胞研究概况（评述）3

小鼠骨髓造血干细胞（CFU-S、CFU-C）的性质和功能研究7

造血干细胞与免疫活性细胞的沉降特性13

造血干细胞的浓集及其功能研究23

胎肝造血干细胞的移植特性31

脾结节生成细胞群的不均一性研究38

胎肝造血干细胞在扩散盒培养条件下移植特性的变化46

不同来源CD34+CD38+和CD34+CD38-细胞群造血性能的不均一性研究54

人胎肝造血干细胞增殖刺激物的分离及鉴定59

脐血CD34+细胞扩增和改善其移植效率的实验研究64

小鼠造血干细胞增殖与分化性能的研究69

造血重建小鼠骨髓中成纤维细胞的起源76

干细胞因子促进造血细胞与纤连蛋白的粘附82

人脐血间充质干细胞体外扩增和向神经元样细胞定向诱导分化的研究88

微载体悬浮培养成人骨髓间充质干细胞95

人Delta-like-1胞外区在CHO细胞的表达纯化及对造血祖细胞的扩增作用105

激活Notch信号通路促进骨髓间充质干细胞向成骨细胞分化112

Studies on the property and transplantation of haemopoietic stem cells from peripheral blood119

Kinetic studies of haemopoietic progenitor cells（CFU-c）and T-lymphocytes in the liver of human foetus129

Characteristics of proliferation and differentiation of spleen colony-forming cells from bone marrow137

Cryopreservation of hematopoietic cells145

Comparative studies on the characteristics of proliferation and differentiation of spleen colony-forming cells152

Characteristics of hemopoietic stem cells determined by single spleen colony transplantation162

Kinetics of the clonal proliferation of granulocytes and macrophages in cultures of mouse bone marrow cells as supported by two distinct types of colony-stimulating factors170

Advances in experimental studies and clinical application of fetal liver cells182

Translation of poly（A）+mRNA encoding CFU-S proliferation stimulator of human fetal liver origin in Xenopus laevis oocytes189

Comparison of the hemopoietic growth factors produced during in vitro culture of murine and human fetal liver cells195

A correlation study on molecular evolution of hemopoietic growth factors and development of hemopoietic cells204

Molecular evolution of cytokines and their receptors208

Human primitive hematopoietic progenitor cells are more enriched in CD34+CD38-population than in CD34+CD38+population215

第二部分 放射生物学225

造血干细胞在放射医学研究中的意义（评述）225

脱氧核糖核蛋白中脱氧核糖核酸的辐射敏感性233

造血干细胞辐射敏感性的比较244

多向性造血干细胞辐射敏感性药物影响和活存曲线的分析253

自体外周血干细胞移植对急性放射病狗的疗效观察257

同种Beagle狗循环血造血干细胞移植治疗急性放射病的实验研究261

5 Gy60Coγ线照射后小鼠骨髓中红系祖细胞损伤和恢复的动态观察266

低剂量率γ线连续照射后造血损伤与恢复的长期观察271

哺乳类细胞受照射后DNA损伤修复中的“二次损伤”现象及其机理初探275

一例重度骨髓型急性放射病的临床报告280

OSM对黑色素瘤的抑制作用与基因-放射治疗效果291

白藜芦醇的辐射防护及其分子机理的研究297

Haemopoietic stem-cell kinetics during continuous irradiation301

The nature of radiation damage of haemopoietic stem cells under continuous irradiation at low dose rate311

Studies of haemopoietic stem cells and microenvironment in chronic irradiated mice321

Kinetic studies of radiation damage and recovery of murine haemopoietic stem cells during and after continuous irradiation at low dose rate332

第三部分 肝细胞生成素345

The mechanism of clinical effectiveness of human fetal liver cell transfusion（评述）345

人胎肝中肝细胞生长因子生物活性的研究349

人胎肝中肝细胞生长因子poly（A）+mRNA在非洲爪蟾卵母细胞内的翻译355

人胎肝中肝细胞生长因子的纯化及某些生物学特性的研究361

肝细胞生长因子治疗小鼠中毒性肝炎的实验研究367

新生牛肝脏生长因子的纯化及其理化特性与生物活性372

人胎肝细胞裂解液治疗慢性活动性肝炎伴肝硬化的疗效观察377

纯化肝细胞生长因子对肝癌细胞的增殖刺激作用378

重组人肝再生增强因子抗肝损伤作用382

肝部分切除后肝再生增强因子信使核糖核酸（mRNA）表达增强385

Hepatokine and methods for its use388

Human hepatic stimulator substance：a product of gene expression of human fetal liver tissue399

Partial isolation and identification of hepatic stimulator substance mRNA extracted from human fetal liver408

Protective effect of recombinant human augmenter of liver regeneration on CCl4-induced hepatitis in mice413

第四部分 低分子抑瘤物427

揭开人体中天然肿瘤抑制物的奥秘（评述）………………………………………………（423)人胎肝低分子天然抑瘤物在体外骨髓净化中的应用427

人胎肝细胞分泌的低分子抑瘤物对白血病细胞的抑制作用430

人胎肝低分子天然抑瘤物体外培养净化自体骨髓移植治疗急性白血病436

邻苯二甲酸正丁酯体外净化白血病细胞的实验研究439

邻苯二甲酸正丁酯诱导白血病细胞系HL-60、U937细胞程序性死亡442

人胎肝中低分子抑瘤物的分离纯化、结构鉴定及其对肿瘤细胞生长的选择性抑制作用446

邻苯二甲酸正丁酯净化自体骨髓移植治疗白血病451

人胎肝中两种抑制肿瘤细胞生长活性成分的分离鉴定及功能研究456

Effects of human fetal liver extract on the growth of HL-60 cells462

Studies on the suppression of HL-60 cell growth in vitro by low molecular weight suppressor of human fetal liver origin470

Preferential suppression of low molecular weight natural tumor suppressor of human fetal liver origin on the growth of leukemic cells in vitro478

Experimental treatment of L-801 myeloid leukemia mice with low molecular weight natural tumor suppressor of human fetal brain origin487

A new pharmacological function of phthalate ester plasticizer493

A new pharmacological activity of dibutyl phthalate（DBP） on selective elimination of tumor cells from bone marrow498

Purging effect of dibutyl phthalate on leukemic cells associated with apoptosis503

Purging effect of dibutyl phthalate on leukemia cells involves fas independent activation of caspase-3/CPP32 protease509

第五部分 基因治疗519

基因治疗药物向临床走来（评述）519

小鼠免疫共刺激信号B7-2 cDNA的克隆与序列测定523

具重复Kringle结构分子系列缺失体和嵌合体的构建方法及验证528

腺病毒介导多基因在肺癌细胞中的表达及致凋亡效应535

p14ARF基因抑制肿瘤细胞增殖的研究542

质粒pcDNA3-HGF的大规模纯化制备研究549

腺病毒介导多基因对肝癌细胞凋亡的诱导作用554

重组腺病毒介导人野生型p53，GM-CSF和B7-1基因对肿瘤化疗耐药细胞生物学行为的影响558

共表达人p53、GM-CSF和B7-1基因的重组腺病毒的构建569

腺病毒介导的人野生型p53、GM-CSF和B7-1基因转移诱导肝癌细胞的生长抑制和凋亡577

基因枪在大鼠闭塞性血管病基因治疗中的应用581

质粒pUDKH刺激鸡胚绒膜尿囊膜血管生成效应的研究587

质粒治疗肢体动脉闭塞性疾病的安全性分析593

腺病毒载体介导肝细胞生长因子基因对大鼠心肌缺血的基因治疗597

肝细胞生长因子促进膀胱平滑肌创伤愈合的研究603

肝细胞生长因子基因转染促进伤口愈合及预防瘢痕形成的实验研究607

质粒pUDKH基因治疗大鼠肢体动脉闭塞病的实验研究610

人肝细胞生长因子基因治疗犬肢体动脉闭塞病616

携带人肝细胞生长因子基因的重组腺病毒对大鼠局灶性脑缺血治疗的实验研究622

腺病毒携带p53、GM-CSF、B7-1基因转染乳腺癌细胞诱导抗肿瘤免疫的研究627

携带肝细胞生长因子基因的重组腺病毒对犬心肌缺血的基因治疗630

腺病毒介导人肝细胞生长因子对大鼠皮层神经元损伤的保护作用637

TTransfer of p14ARF gene in drug-resistant human breast cancer MCF-7/Adr cells inhibits proliferation and reduces doxorubicin resistance644

Effect on biological behavior of chemotherapy-resistant tumor cells by human wild-type p53，GM-CSF and B7-1 genes via recombinant adnovirus654

Co-transfer of human wild-type p53 and granulocyte-macrophage colony-stimulating factor genes via recombinant adenovirus induces apoptosis and enhances immunogenicity in laryngeal cancer cells668

Growth suppression and immunogenicity enhancement of Hep-2 or primary laryngeal cancer cells by adenovirus-mediated co-transfer of human wild-type p53，granulocyte-macrophage colony-stimulating factor and B7-1 genes679

Treatment of myocardial ischemia with bone marrow-derived mesenchymal stem cells overexpressing hepatocyte growth factor690

Synergetic anticancer effect of combined quercetin and recombinant adenoviral vector expressing human wild-type p53，GM-CSF and B7-1 genes on hepatocellular carcinoma cells in vitro703

Effect of human hepatocyte growth factor on promoting wound healing and preventing scar formation by adenovirus-mediated gene transfer715

Gene therapy for pathological scar with hepatocyte growth factor mediated by recombinant adenovirus vector723

Sphingosine kinase activation regulates hepatocyte growth factor induced migration of endothelial cells732

增强自主创新能力、加快发展现代医药产业（评述）749

第六部分 药物应用及专利公开说明749

中国人γ-干扰素cDNA在大肠杆菌中的高效表达752

高效液相色谱法分析小鼠血浆中贝母乙素及其药代动力学研究757

大黄非蒽醌类成分的研究761

二苯乙烯类化合物El对2型糖尿病大鼠血清游离脂肪酸组成的影响765

二苯乙烯类化合物E1的降糖作用及其机制的初步研究772

二苯乙烯类化合物E1对2型糖尿病大鼠心肌病变的影响777

柱色谱-紫外分光光度法测定广金钱草中总黄酮的含量782

葛香感冒胶囊质量标准的研究787

HPLC制备河套大黄中多羟基芪类化合物Rhapontin793

Screening of common traditional Chinese drugs for reversing muhidrug resistance of KBV200 in vitro797

Three eoumarins from seed of Cnidium monnieri and their multidrug resistance reversal effects802

Hepatokine and methods for its use806

一种新的人神经分化因子806

一种重组腺病毒及其在肿瘤治疗中的应用806

携带人肝细胞生长因子基因的重组载体及其在缺血性疾病中的应用806

一类新型降低血糖化合物807

一种新型人蛋白分泌相关因子807

一种重组腺病毒及其在纤维性疾病防治中的应用807

一种重组质粒及其在疾病防治中的应用808

一种新型人神经细胞突起生长诱向因子808

一种新的抗肿瘤活性物质808

一类新的苯并噻吩类化合物在防治妇女绝经后综合征和其他与雌激素相关809

疾病中的用途809

人骨髓和脐带血间充质干细胞体外分离扩增和向神经细胞定向诱导的方法809

二苯乙烯类化合物制备以及它们在治疗和预防糖尿病中的应用809

新的取代庚酸衍生物治疗支气管哮喘和慢性阻塞性肺病809

用于加速创伤修复及防治并发症的方法810

一种黄酮类化合物治疗尿石症、胆石症的用途及其制备工艺810

亲和保护活性区进行化学修饰蛋白质的方法、产品及用途810

高效表达重组水蛭素及其生产方法810

苯乙烯类化合物在制备用于治疗和预防糖尿病或与逆转录病毒有关疾病的药物中用途811

Use of stilbene compounds in preparing medicaments for treating or preventing diabetes and diseases associated with retrovirus811

一类溶栓抗凝双功能融合蛋白及应用811

一种重组腺病毒在脑缺血、创伤修复和组织粘连治疗中的应用812

磺酰苯胺类衍生物及其医药用途812

Fused protein with the fanction of both hemolysis and anticoagulation and the use of it812

酶性止血剂拮抗水蛭素出血作用的用途813

致谢814