全国高等学校教材 细胞生物学实验教程 双语版2025|PDF|Epub|mobi|kindle电子书版本百度云盘下载

全国高等学校教材 细胞生物学实验教程 双语版PDF格式电子书版下载

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实验一 光学显微镜的结构和使用1

Experiment 1 The Structure and the Usage of an Optical Microscope5

实验二 细胞形态和细胞器的观察10

Experiment 2 Observation of Cell Morphology and Organelles13

实验三 显微测量16

Experiment 3 Microscopic Measurement18

实验四 细胞膜的通透性20

Experiment 4 Permeability of Cell Membrane22

实验五 聚乙二醇（PEG）诱导的细胞融合25

Experiment 5 Cell Fusion Induced by Polyethylene Glycol（PEG）27

实验六 线粒体的活体染色29

Experiment 6 Supravital Staining of Mitochondria31

实验七 动植物细胞骨架的制备和观察33

Experiment 7 The Preparation and Observation of Cytoskeleton of Animal Cells and Plant Cells35

实验八Feulgen染色法测定DNA38

Experiment 8 Identify DNA by Feulgen Staining40

实验九 毛囊细胞核仁组织区银染43

Experiment 9 Silver Staining Technique for Nucleolus Organizer Region of Hair Follicle Cells45

实验十 人类染色体标本制备48

Experiment 10 Human Chromosome Preparation50

实验十一 人类染色体常规核型分析52

Experiment 11 Analysis of Conventional Human Chromosome Karyotype55

实验十二动、植物细胞有丝分裂的观察59

Experiment 12 Observation of Mitosis in Animal Cells and Plant Cells61

实验十三 蝗虫精巢减数分裂的观察64

Experiment 13 Observation of Meiosis in the Grasshopper Testis67

实验十四 动物细胞原代培养71

Experiment 14 Primary Culture of Animal Cells73

实验十五 细胞的传代培养75

Experiment15Cell Subculture77

实验十六 细胞计数和死活细胞的鉴定80

Experiment 16 Cell Counting and Identification of Dead and Alive Cells83

实验十七MTT和CCK-8检测法86

Experiment 17 MTT and CCK-8Detection89

实验十八 植物愈伤组织的诱导93

Experiment 18 Induction of Plant Callus96

实验十九TRIzol法提取RNA和DNA100

Experiment 19 RNA and DNA Extraction Using TRIzol Reagent103

实验二十 琼脂糖电泳法鉴定DNA106

Experiment 20 Identify DNA with the Method of Agarose Gel Electrophoresis108

实验二十一 聚合酶链式反应（PCR）110

Experiment 21 Polymerase Chain Reaction（PCR）113

实验二十二 脂质体介导转染法116

Experiment 22 Liposome-mediated Transfection118

实验二十三DNA重组技术120

Experiment 23 DNA Recombination Technology125

实验二十四 蛋白质聚丙烯酰胺凝胶电泳及western blot分析130

Experiment 24 Polyacrylamide Gel Electrophoresis And Western Blot for Proteins134