田波病毒学文选2025|PDF|Epub|mobi|kindle电子书版本百度云盘下载

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第一篇 亚病毒3

第一章 亚病毒的研究进展3

亚病毒——病毒学的一个新分支5

第二章 病毒卫星RNA及其在病毒病防治上的应用13

黄瓜花叶病毒组的卫星RNA15

植物病毒卫星RNA及其在病毒病生物防治上的应用23

卫星RNA作为黄瓜花叶病毒的生防因子26

卫星RNA作为黄瓜花叶病毒病生防因子的研究30

植物病毒卫星RNA生防制剂CMV-S52防治番茄病毒病36

烟台市应用弱毒疫苗S51和N14防治辣椒花叶病毒病的试验38

带卫星RNA黄瓜花叶病毒保护接种的植物中病毒积累与基因组RNA合成及病状表达40

抗黄瓜花叶病毒的卫星RNA互补DNA转基因番茄47

Control of Two Seed-Borne Virus Diseases in China by The Use of Protective Inoculation48

Double-Stranded Cucumovirus Associated RNA 5:Experimental Analysis of Necrogenic and Non-necrogenic Variants by Temperature-Gradient Gel Electrophoresis52

Satellite RNA for The Biocontrol of Plant Disease61

High Resistance to Cucumber Mosaic Virus Conferred by Satellite RNA and Coat Protein in Transgenic Commercial Tobacco Cultivar G-14073

Resistance of Tomato Infected with Cucumber Mosaic Virus Satellite RNA to Potato Spindle Tuber Viroid82

Viroid-Like RNA Encapsidated in Lucerne Transient Streak Virus89

Multiplication of Velvet Tobacco Mottle Virus in Nicotiana clevelandii Protoplasts is Resistant to α-Amanitin93

Plant Resistance to Fungal Diseases Induced by the Infection of Cucumber Mosaic Virus Attenuated by Satellite RNA99

Rapid Production and Field Testing of Homozygous Transgenic Tobacco Lines with Virus Resistance Conferred by Expression of Satellite RNA and Coat Protein of Cucumber Mosaic Virus104

Controlling Mosaic Virus Diseases Under Field Conditions Using Multiple Gene Strategies in Transgenic Plants113

第三章 类病毒的研究127

类病毒研究进展和展望129

用互补DNA分子杂交和聚丙烯酰胺凝胶电泳测定我国菊花矮化类病毒138

苹果锈果病组织中发现的环状类病毒RNA142

我国菊花褪绿斑驳类病毒鉴定143

苹果锈果病（ASSD）类病毒的侵染性150

接种外表健康的梨树芽片诱发苹果锈果病类病毒155

苹果锈果类病毒的温度梯度凝胶电泳159

高抗马铃薯纺锤形块茎类病毒的转基因马铃薯细胞内核酶转录产物的分布164

Pear as A Natural Host of Apple Scar Skin Viroid168

Ribozyme-Mediated High Resistance Against Potato Spindle Tuber Viroid In Transgenic Potatoes171

第四章 朊病毒的研究179

朊病毒的研究进展181

免疫印迹法检测牛海绵状脑病和羊瘙痒病188

High-Level Expression and Secondary Structure Analysis of The Bovine Mature Prion Protein193

Conformational Change of Glutathione-S-Transferase by Its Co-Expression with Prion Domain of Yeast Ure2p197

On-Column Purification and Refolding of Recombinant Bovine Prion Protein:Using Its Octarepeat Sequences as A Natural Affinity Tag203

An Engineered PrPsc-Like Molecule From The Chimera of Mammalian Prion Protein and Yeast Ure2p Prion-Inducing Domain210

Interaction of Doppel with The Full-Length Laminin Receptor Precursor Protein216

In vitro Self-Propagation of Recombinant PrPsc-Like Conformation Generated In The Yeast Cytoplasm223

Bovine PrPc Directly Interacts with αβ-Crystalline230

Aggregation of Prion Protein with Insertion Mutations is Proportional to The Number of Inserts238

第二篇 植物病毒253

第一章 病毒和高温对马铃薯花叶型退化的影响253

马铃薯块茎发芽条件对芽内X-病毒浓度的影响255

马铃薯在温度条件影响下对花叶病毒抵抗力的改变与种薯退化关系的证据265

在马铃薯退化问题上耐病性变化观念的形成和验证280

西藏高原的生态条件对于马铃薯花叶型退化的影响284

病毒和高温在马铃薯花叶型退化中的作用289

油乳剂对蚜虫传染非持久性植物病毒的抑制作用296

Loss of Tolerance to Mosaic Viruses as The Cause of Potato Degeneration303

Potato Degeneration Research in China312

马铃薯种薯生产的研究Ⅰ．用茎尖培养方法生产马铃薯无病毒原种316

马铃薯种薯生产的研究Ⅱ．几种血清学方法对马铃薯病毒X诊断效果的比较322

马铃薯无病毒留种体系的研究327

第二章 植物双生病毒331

植物双生病毒研究进展333

烟草曲叶双生病毒的研究Ⅰ．广西分离物的生物学特性和血清学反应343

烟草曲叶双生病毒分子进化的初步研究348

番茄曲叶病及其血清学和PCR测定352

一种非洲番茄黄化曲叶病毒DNA的克隆356

Chinese Squash Leaf Curl Virus:A New Whitefly-Transmitted Geminivirus363

Chinese Tomato Yellow Leaf Curl Virus—A New Species of Geminivirus370

第三章 植物病毒弱毒疫苗377

植物病毒弱毒系及其应用Ⅰ．烟花叶病毒番茄株弱毒系的诱变和性质的研究379

植物病毒弱株系及其应用Ⅱ．烟花叶病毒番茄株弱株系N11对番茄的保护作用384

植物病毒弱株系及其应用Ⅲ．烟花叶病毒番茄株弱毒疫苗N14的安全性测定388

浸根法对番茄接种弱毒株（N14）获得的效果396

延边地区烟草病毒种类及病毒病的防治398

油菜花叶病毒15号和菸花叶病毒间干扰作用的研究405

用DNA杂交技术测定烟草花叶病毒与长叶车前花叶病毒的关系411

番茄花叶病毒及其弱毒N14株RNAs在兔网织细胞裂解液体系中翻译产物的比较研究415

相关和不相关病毒mRNA在兔网织细胞裂解液中的竞争性翻译418

干扰素的功能表达机制424

酵母校正tRNA的提取及其对番茄花叶病毒增殖的抑制作用427

Taxonomy of Several Tobamoviruses from China as Determined by Molecular Hybridization Analysis with Complementary DNA434

Classification of Several Tobamoviruses Isolated in China on the Basis of The Amino Acid Composition of Their Virion Proteins440

Double-Stranded Viral RNA Content in Tobacco Leaves Infected with Virulent and Avirulent Isolates of Tomato Mosaic Virus444

第四章 禾本科作物病毒及其克隆与表达449

水稻条纹叶枯病毒分子生物学的研究——外壳蛋白分子量、氨基酸组成和N末端451

小麦丛矮病毒的研究454

虎尾草条纹花叶病毒DNA克隆对小麦的农杆菌侵染461

水稻条纹叶枯病毒基因组含vRNA2 ORF片段的克隆、序列分析及其在原核中的表达465

水稻普矮病毒基因组第十片段cDNA的克隆及其体外转录469

水稻矮缩病毒大外膜蛋白基因的合成、克隆和序列分析474

水稻矮缩病毒小外壳蛋白基因的合成、克隆和序列分析478

Nucleotide Sequence of Maize Dwarf Mosaic Virus Capsid Protein Gene and Its Expression in Escherichia coli482

Resistance to Rice Stripe Virus Conferred by Expression of Coat Protein in Transgenic Indica Rice Plants Regenerated from Bombarded Suspension Culture488

The Study of Molecular Biology of Rice Stripe Virus（Ⅰ）——Molecular Weight,Amino Acid Composition and Property of Coat Protein497

Infection of Barley Protoplasts with Barley Stripe Mosaic Virus Detected and Assayed by Immunoperoxidase500

Ribozyme-Mediated Resistance to Rice Dwarf Virus and The Transgene Silencing in The Progeny of Transgenic Rice Plants505

第五章 其他植物病毒及其克隆与表达515

构建具侵染性的花椰菜花叶病毒克隆517

芜青花叶病毒外壳蛋白基因的克隆和表达521

番木瓜环斑病毒外壳蛋白基因的构建524

一个黄瓜花叶病毒强株系的外壳蛋白基因的合成、克隆、序列分析和表达528

哈密瓜花叶病流行的研究533

引起哈密瓜坏死的两种病毒的分离及鉴定537

油菜花叶病毒（YMV6）部分提纯及其若干性质的初步研究543

用酶标抗体检测植物细胞和原生质体内的烟草花叶病毒549

抗黄瓜花叶病毒单克隆抗体细胞株的建立及其抗体鉴定555

用A蛋白夹层酶联免疫吸附法对黄瓜花叶病毒的血清学鉴定561

Cucumber Mosaic Virus from Cornflower in China566

Discovery and Demonstration of Small Circular DNA Molecules Derived from Chinese Tomato Yellow Leaf Curl Virus570

第三篇 医学与动物病毒577

第一章 乙肝病毒及其T细胞免疫与热休克蛋白96的佐剂作用577

四环素调控表达系统调节EGFP和HBV前核心蛋白在哺乳动物细胞中表达579

HBV-Specific Peptide Associated with Heat-Shock Protein gp96585

Three-Step Purification of gp96 from Human Liver Tumor Tissues Suitable for Isolation of gp96-Bound Peptides588

Significant Correlation Between Expression Level of Hsp gp96 and Progression of Hepatitis B Virus Induced Diseases596

New Serum Biomarkers for Detection of HBV-Induced Liver Cirrhosis Using SELDI Protein Chip Technology604

Generation of Murine CTL by A Hepatitis B Virus-Specific Peptide and Evaluation of the Adjuvant Effect of Heat Shock Protein Glycoprotein 96 and Its Terminal Fragments609

Enhancement of Humoral Immune Responses to HBsAg By Heat Shock Protein gp96 and Its N-Terminal Fragment in Mice622

Hepatitis B Virus Core Antigen Epitopes Presented by HLA-A2 Single-Chain Trimers Induce Functional Epitope-Specific CD8+ T-Cell Responses In HLA-A2.1/Kb Transgenic Mice631

A Mutant HBs Antigen(HBsAg)183-191 Epitope Elicits Specific Cytotoxic T Lymphocytes in Acute Hepatitis B Patients641

The High Prevalence of the 127 Mutant HBcAg 18-27 Epitope in Chinese HBV-Infected Patients and Its Cross-Reactivity with the V27 Prototype Epitope650

Enhancing The potency of HBV DNA Vaccines Using Fusion Genes of HBV-Specific Antigens and the N-Terminal Fragment of gp96660

PD-1 and PD-L1 Upregulation Promotes CD8+ T-Cell Apoptosis and Postoperative Recurrence in Hepatocellular Carcinoma Patients678

Skewed Ratios Between CD3+ T Cells and Monocytes are Associated with Poor Prognosis in Patients with HBV-related Acute-on-chronic Liver Failure690

第二章 其他医学与动物病毒701

Following the Rule:Formation of the 6-Helix Bundle of the Fusion Core from Severe Acute Respiratory Syndrome Coronavirus Spike Protein and Identification of Potent Peptide Inhibitors703

Six-Helix Bundle Assembly and Analysis of the Central Core of Mumps Virus Fusion Protein710

Both Heptad Repeats of Human Respiratory Syncytial Virus Fusion Protein are Potent Inhibitors of Viral Fusion718

The Antibodies Directed Against N-Terminal Heptad-repeat Peptide of hRSV Fusion Protein and Its Analog-5-Helix Inhibit Virus Infection in vitro727

Design and Characterization of Human Respiratory Syncytial Virus Entry Inhibitors735

Analysis of a Point Mutation in H5N1 Avian Influenza Virus Hemagglutinin in Relation to Virus Entry into Live Mammalian Cells744

Evaluation of Glu11 and Gly8 of The H5N1 Influenza Hemagglutinin Fusion Peptide in Membrane Fusion Using Pseudotype Virus and Reverse Genetics755

A Dual Reporter Gene Based System to Quantitate the Cell Fusion of Avian Influenza Virus H5N1766

Structural Characterization of Mumps Virus Fusion Protein Core774

Rattonal Design of Highly Potent HIV-1 Fusion Inhibitory Proteins:Implication for Developing Antiviral Therapeutics782

Screening for CD8 Cytotoxic T Lymphocytes Specific for Gag of Human Immunodeficiency Virus Type 1 Subtype B'Henan Isolate from China and Identification of Novel Epitopes Restricted by the HLA-A2 and HLA-A11 Alleles790

Recombinant Protein of Heptad-Repeat HR212,A Stable Fusion Inhibitor with Potent Anti-HIV Action in vitro800

Glycoprotein 96-Mediated Presentation of Human Immunodeficiency Virus Type 1(HIV-1)-Specific Human Leukocyte Antigen Class I-Restricted Peptide and Humoral Immune Responses to HIV-1 p24811

Effect of RNA Oligonucleotide Targeting Foxo-1 on Muscle Growth in Normal and Cancer Cachexia Mice821

Myostatin Antisense RNA-Mediated Muscle Growth in Normal and Cancer Cachexia Mice831

A Retrovirus-Based System to Stably Silence GDF-8 Expression and Enhance Myogenic Differentiation in Human Rhabdomyosarcoma Cells840

Inhibition of Atrogin-1/MAFbx Expression by Adenovirus-Delivered Small Hairpin RNAs Attenuates Muscle Atrophy in Fasting Mice852

Circulating microRNAs in Hepatitis B Virus-Infected Patients868

Identification of the Myelin Oligodendrocyte Glycoprotein as a Cellular Receptor for Rubella Virus880

贺信897

中国科学院病原微生物与免疫学重点实验室简介899

后记903