可变重力条件下神经元系统中的自组织和斑图动力学 空间条件下的生命科学（英文版）2025|PDF|Epub|mobi|kindle电子书版本百度云盘下载

可变重力条件下神经元系统中的自组织和斑图动力学 空间条件下的生命科学（英文版）PDF格式电子书版下载

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Chapter 1 Introduction1

1.1 Historical remarks1

1.1.1 Gravitational research1

1.2 Excitable media and their control by small external forces2

1.3 Waves and oscillations in biological systems3

1.4 Book layout4

References5

Chapter 2 Gravity7

2.1 Physical remarks7

2.2 Perception of gravity by living systems8

References9

Chapter 3 Basic Structure of Neuronal Systems11

References18

Chapter 4 Platforms for Gravitational Research19

4.1 Microgravity platforms20

4.1.1 Short term platforms21

4.1.2 Long term platforms31

4.1.3 Magnetic levitation34

4.2 Removing orientation36

4.2.1 Clinostats37

4.2.2 Random positioning machine37

4.3 Macro-gravity platforms38

4.3.1 Centrifuge39

References41

Chapter 5 A Model Systems for Gravity Research:The Belousov-Zhabotinsky Reaction43

5.1 Setup for the Belousov-Zhabotinsky experiments44

5.2 Preparation of gels for the Belousov-Zhabotinsky reaction46

5.3 Data evaluation46

References55

Chapter 6 Interaction of Gravity with Molecules and Membranes57

6.1 Bilayer experiments58

6.1.1 Hardware for the Microba mission60

6.1.2 Hardware for the drop-tower62

6.1.3 Hardware for parabolic flights62

6.1.4 Hardware for laboratory centrifuge64

6.1.5 Experimental results64

6.2 Patch-clamp experiments69

6.2.1 Principles of patch-clamp experiments69

6.2.2 Hardware for the drop-tower71

6.2.3 First hardware for parabolic flights75

6.2.4 For the drop-tower76

6.2.5 First parabolic flight experiment77

6.2.6 Second hardware for parabolic flights78

6.2.7 Second parabolic flight experiment86

6.2.8 First results and future perspectives89

References92

Chapter 7 Behavior of Aetion Potentials Under Variable Gravity Conditions95

7.1 Introductory remarks95

7.2 Materials and methods99

7.3 Isolated leech neuron experiments100

7.4 Earthworm and nerve fiber experiments(rats and worms)102

7.5 Discussion105

References108

Chapter 8 Fluorescence and Light Scatter Experiments to Investigate Cell Properties at Microgravity111

8.1 Fluorescence measurements to determine calcium influx and membrane potential changes111

8.1.1 Intracellular calcium concentration experiments112

8.1.2 Membrane potential experiments113

8.2 Light scatter experiments to determine changes in cell size114

8.2.1 Static light scatter115

8.2.2 Dynamic light scatter118

References121

Chapter 9 Spreading Depression:A Self-organized Excitation Depression Wave in Different Gravity Conditions123

9.1 The retinal spreading depression124

9.2 Gravity platforms used for retinal spreading depression experiments126

9.2.1 Methods127

9.2.2 Experiment setup and protocol for spreading depression experiments in parabolic flights128

9.2.3 Experiment setup and protocol for spreading depression experiments on TEXUS sounding rocket129

9.2.4 Setup and protocol for spreading depression experiments in the centrifuge132

9.2.5 Data analysis132

9.3 Results134

9.3.1 Spreading depression experiments in parabolic flights and in the centrifuge134

9.3.2 Spreading depression experiments on sounding rockets and in the centrifuge136

9.3.3 Determination of latency of spreading depression waves in the centrifuge138

9.3.4 Summary of all spreading depression experiments on different gravity platforms139

9.4 Discussion140

9.4.1 Comment on different gravity platforms141

References142

Chapter 10 The Brain Itself in Zero-g145

10.1 Methods147

10.1.1 Slow cortical potentials(SCP)148

10.1.2 Classical frequency bands in EEG149

10.1.3 Peripheral psycho physiological parameters150

10.1.4 Protocol and data analysis150

10.1.5 Subjects152

10.1.6 Ethic153

10.2 Results153

10.2.1 Slow Cortical Potentials(SCP)154

10.2.2 Frequency band EEG157

10.2.3 Peripheral stress parameters159

10.3 Discussion161

10.3.1 Slow cortical potentials161

10.3.2 Frequency band EEG162

10.3.3 Peripheral parameters163

10.4 Conclusion163

References164

Chapter 11 Effects of Altered Gravity on the Actin and Microtubule Cytoskeleton,Cell Migration and Neurite Outgrowth167

11.1 Summary167

11.2 Introductory remarks168

11.3 Material and methods169

11.3.1 Cell transfection169

11.3.2 Cell culture and experiments with SH-SY5Y neuroblastoma cells169

11.3.3 Cell migration experiments-Human carcinoma cell lines170

11.3.4 Scratch Migration Assay(SMA)170

11.3.5 Neurite outgrowth experiments-Primary cell culture of embryonic chicken spinal cord neurons170

11.3.6 Imunostaining of cells171

11.3.7 Staining of F-actin171

11.3.8 Microscopy and live imaging171

11.4 Results and discussion172

11.4.1 Effects of altered gravity on actin-driven lamellar protrusion of SH-SY5Y neuroblastoma cells172

11.4.2 Effect of altered gravity on the microtubule cytoskeleton of SH-SY5Y neuroblastoma cells177

11.4.3 Effects of altered gravity on cell migration181

11.4.4 Effects of altered gravity on the intensity and direction of neurite outgrowth182

References184

Chapter 12 Discussion and Perspectives187

References192

Index193