机能学实验指导2025|PDF|Epub|mobi|kindle电子书版本百度云盘下载

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第一章 绪论1

第一节 机能实验学教程的内容、目的和要求1

第二节 实验报告的书写2

第三节 实验室守则4

第二章 机能学实验动物5

第一节 实验动物的基本知识5

第二节 实验动物的麻醉12

第三节 实验动物用药量的确定13

第三章 机能学实验常用仪器15

第一节 微机技术与网络教学15

第二节 RM6240C微机生物信号采集处理系统15

第四章 机能学动物实验的基本操作技术17

第一节 动物的捉拿及不同途径给药17

第二节 坐骨神经—腓肠肌、坐骨神经干标本制备24

第三节 离体蟾蜍心脏标本的制备26

第四节 离体小肠段的制备28

第五节 离体血管标本的制备29

第六节 大鼠离体心脏Langendorff灌流技术30

第七节 家兔活体解剖技术31

第五章 机能学综合实验34

第一节 刺激与肌肉收缩反应的关系34

第二节 心电图描记，期前收缩和代偿间歇，离体蛙心灌流36

第三节 局麻药对兔眼作用的观察42

第四节 家兔动脉血压调节实验43

第五节 水肿的观察及治疗46

第六节 缺氧的观察及治疗48

第七节 氨在肝性脑病发病中的作用50

第八节 药代动力学实验52

第九节 药物作用观察60

第十节 不同途径给药观察及ED50测定63

第十一节 家兔失血性休克68

第十二节 家兔离体小肠段的特性观察及药物影响70

第十三节 尿生成的调节及利尿药作用观察72

第十四节 实验性急性右心衰75

第十五节 病例讨论79

参考文献83

附录84

一、常用实验溶液的成分及配制84

二、常用实验动物的生理常数84

Chapter 1 Introduction88

Section 1 The Content, Purpose and Requirements of Functional Experiments course88

Section 2 The writing of the experimental report89

Section 3 The rules of laboratory92

Chapter 2 Basic knowledge about laboratory animals required in the functional experiments94

Section 1 Basic knowledge about laboratory animals94

Section 2 Anesthesia of laboratory animals102

Section 3 How to calculate the drug dosage to animals104

Chapter 3 Common apparatus used in functional experiments106

Section 1 Computer and local network technique used in teaching106

Section 2 RM6240C channel physiological signal acquiring and processing system106

Chapter 4 Basic operation technology onanimal experiment of functional science109

Section 1 Animal handling and routes of administration109

Section 2 Preparation of sciatic nerve-gastrocnemius muscle and sciatic nerve trunk samples117

Section3 Preparation of isolated toad heart119

Section 4 Preparation of isolated intestinal smooth muscle samples122

Section 5 Preparation of isolated vascular specimens123

Section 6 Langendorff perfusionof isolated rat heart124

Section 7 Anatomical technique ofrabbits125

Chapter 5 Comprehensive experiments of functional science129

Section 1 The relationship between muscle stimulation and muscle contraction129

Section 2 Recording electrocardiography, premature systole, compensatory pause and frog heart perfusion in vitro132

Section 3 The surface anesthesia effect oftetracaine and procaine on rabbit eyes139

Section 4 Functional experiments on blood pressure regulation in rabbits141

Section 5 Observation and treatment of experimental edema in toads145

Section 6 Observation and treatment of different hypoxia in mice147

Section 7 Effects of ammonia on hepatic encephalopathy150

Section 8 Drug metabolism and pharmacokinetics153

Section 9 Therapeutic effects of drug on animal body162

Section 10 Comparing the effects ofpentobarbital sodium on mice by different routes of administration and mensuration of ED50166

Section 11 Rabbit model of acute hemorrhagic shock171

Section 12 Analysis of effects of drugs acting on receptors of the rabbit isolated intestinal smooth muscles174

Section 13 Regulation of urine production and effect of diuretic in rabbits177

Section 14 Acute right heart failure in rabbits and the therapeutics181

Section 15 Case discussion184

References190

Appendix192

1. Preparation and Component of Experimental Solution192

2. Physiological Constants of Laboratory Animals192